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We found no difference between the two groups (Supplementary Fig

Concentrations of the peptide up to 500 ng/mL failed to increase proliferation of mouse C2C12 cells or primary human skeletal muscle myoblasts, failed to inhibit myoblast differentiation, and failed to activate the signaling responses previously attributed to it, even as mature IGF-1 and full-length IGF-1Eb produced robust responses in the same assays

An impairment of this enzyme is associated with an increase in the cellular SAH concentration, which can ultimately result in the inhibition of methyltransferases and is associated with hypomethylation [65]

The Q-PCR cycle conditions were at 95C for 30 s, followed by 40 cycles at 95C for 5 s and at 60C for 30 s