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As polyphenols are poorly bioavailable, they accumulate in the colon and are extensively metabolized by the resident gut bacteria, generating polyphenol-derived microbial metabolites

The low pH of these acids destabilizes the copper peptide complex, potentially reducing its effectiveness and increasing irritation risk

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The mass spectrum in positive ionization mode for complex 2 after 1 min of incubation showed three signals, one at m/z = 613 corresponding to the GSSG and two signals of an iron complex at m/z = 254 and at m/z = 376, corresponding to the [M L] + and [M + Na] + species