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Fortified tortillas made from masa produced through the traditional nixtamalization and wet-milling process, where folic acid was added to nixtamal before milling, contained 15%, 33%, and, in one study, even 80% less folic acid compared to the folic acid amount added to nixtamal (theoretical fortified level)

The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 30 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 2: 20 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles Library 3: 10 M 8-oxo-dGTP, 3 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

Dhaliwal J, Dhaliwal N, Akhtar A, Kuhad A, Chopra K

Camrelizumab in patients with previously treated advanced hepatocellular carcinoma: a multicentre, open-label, parallel-group, randomised, phase 2 trial