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This mechanism may be associated with the inhibition of pro-fibrotic signaling pathways such as Transforming Growth Factor (TGF)-1/Mothers Against Decapentaplegic Homolog (Smad) (Kisseleva and Brenner, 2021)

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Some of the right lungs were harvested and stored at -80C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining