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Repeat the rinsing with PBS three times and then add 300 L of FITC-phalloidin (Solarbio, Beijing, China) solution, incubate for 30 min in the dark, then wash three times with PBS, Add DAPI (4,6-diamidino-2-phenylindole) staining solution (Solarbio, Beijing, China) for 10 min and seal with glycerol

The iron addiction of tumor cells determines that the iron demand is more than that of healthy cells, which leads to cell death due to ferroptosis, mainly depending on the accumulation of iron ions, the metabolism of lipid peroxides and the regulation of related enzymes [33]

Do not use Zepbound if you or any of your family have ever had a type of thyroid cancer called medullary thyroid carcinoma (MTC) or if you have an endocrine system condition called Multiple Endocrine Neoplasia syndrome type 2 (MEN 2)