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The RNA concentration and purity were checked at 260 nm and 280 nm using Infinite M200 PRO Multimode Microplate Reader (Tecan)

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286 In the model of necrotizing crescentic glomerulonephritis induced by anti-neutrophil cytoplasmic autoantibodies, mice that underwent bone marrow transplantation from C5aR1-deficient donors exhibited a marked reduction in the number of neutrophils infiltrating the glomeruli