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Therefore, even in the presence of higher PGC-1 gene expression as a result of mTOR hyperactivity during early AMD stages, a reduction in PGC-1 activity in later stages could ultimately affect mitochondrial turnover and biogenesis causing increased ROS production and cell death

Differences were observed between the two groups for more than 100 metabolites, and 12 differentially identifiable metabolites were identified (Supplementary Table 2 and Supplementary Figure 2G)

Because the skin is the protective barrier to the environment, it can receive direct oxidative damage in the epidermis

These tissue sections were deparaffinized in graded xylene, rehydrated in graded alcohol solutions, and then incubated in antigen retrieval buffer (Roche, Basel, Switzerland) at 37 C for 30 min