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Three primary forms of autophagy, macroautophagy, microautophagy, and molecular chaperone-mediated autophagy, are mediated by lysosomes, which are important organelles for the breakdown of proteins (Mizushima and Levine, 2020)

Firstly, CD206 was employed as the M2 macrophage marker to confirm macrophage polarization status using immunofluorescence staining performed under inflammatory stimuli (LPS and IFN-)