Patients with Barretts esophagus and confirmed persistent low-grade dysplasia are at increased risk for progression to neoplasia
For double conditional knockouts (dKO), we transfected AID-tagged expression vectors (pAID1.1_CENPC-AID_Puro R or pAID1.2_CENP-C-mAid-EGFP_BS R for CENP-C KO, pAID1.1_KNL2-AID_Puro R for KNL2 KO) along with corresponding sgRNA vectors (pX330_CENP-C or pX330_KNL2 and pX330_Ori) into previously established HJURP-cKO (Perpelescu et al, 2015), KNL2-cKO (Hori et al, 2017), or CENP-C-cKO (CENP-C-mAid-mCherry) cells and selected with 0.5 g/ml Puromycin (TAKARA) for Puro R or 10 g/ml Blasticidin S hydrochloride for BS R to generate CENP-C/HJURP-dKO, KNL2/HJURP-dKO, or CENP-C/KNL2-dKO cell lines
This strategy, explored over the last two decades, has recently been successful using GST-activated nitrogen mustard (TLK286) and GT-activated arsenic-based (GSAO and Darinaparsin) prodrugs confirming the potential of GSH-conjugates as anticancer drugs
Infusionen ermglichen es, Wirkstoffe mit hoher Bioverfgbarkeit direkt in den Blutkreislauf zu bringen