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Figure 2 We finally performed WB analysis on the same culture supernatants and bacterial lysates by using the serum of a mouse immunized with rMetE, and also in this case, the NTM supernatants and bacterial lysates were negative for the presence of MetE protein ( Figure 2E ): the absence of a positive signal for MetE in NTM strains, with both mAb M51 and polyclonal antibody anti-MetE, may indicate that the NTM are unable to express MetE protein, unlike Mtb and BCG

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However, not everyone converts synthetic forms efficiently, particularly those with higher metabolic stress, liver strain, or certain genetic variations including the MTHFR mutation