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In healthy people, 90% or more of total glutathione should be GSH, and GSH:GSSG ratios of less than 9:1 indicate oxidative stress

J Clin Invest 120(9):33773388

Subsequently, sections were stained with Hematoxylin and Eosin (HE), Manson-Trichrome (MT), and Alcian-Blue (AB) according to the manufacturer's instructions and studied under the microscope