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However, such exceptionally high and industrially relevant riboflavin levels are usually not encountered amongst spontaneous riboflavin-producing lactobacilli 183

Specific volumes (5 ml) of aliquots were removed at predetermined time intervals (0.5, 1, 2, 4, 6, 8, 12, and 24 h)

With the insulin syringe, draw the amount corresponding to your chosen final concentration (see table below)

It is possible to repeat these four steps to remove the preceding PLS predictors and add additional PLS predictors, but we choose to consider only one potential PLS predictor for the sake of keeping our model as simple and interpretable as possible and because this methodology is prone to overfitting when multiple PLS components are retained