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Targeting Metabolism, Insulin Resistance, and Diabetes to Treat Nonalcoholic Steatohepatitis

For GSH depletion, BSO was added at the final concentration of 120 M

However, recent studies in metabolically competent HepaRG cell lines [23] and primary human hepatocytes [66] have confirmed that exposure to high levels of APAP result in GSH depletion, APAP protein adduct formation in mitochondria and organelle dysfunction accompanied by oxidant stress, resulting in necrotic cell death in human cells as demonstrated earlier in mouse models

G93A-SOD1 epSPC-generated neurospheres exhibited a reactive glial phenotype, as shown by GFAP immunoreactivity that was higher than in cells from age-matched control animals