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The cells were cultured in RPMI1640 (FUJIFILM Wako Pure Chemical Corporation), supplemented with 10% heat-inactivated fetal calf serum (Capricorn Scientific), penicillin (100 U/ml) and streptomycin (100 g/ml) (FUJIFILM Wako Pure Chemical Corporation) and incubated at 37C in 95% humidified air with 5% CO2

The supplement contained biotin, probiotics, vitamin E, zinc, and nicotinamide, with the mens formulation containing beta sitosterol and Boswellia serrata and the womens formulation containing myo-inositol and folic acid

D., Shadel, G

but all these methods are specific to formulation compositions which are far different from those used in common commercial formulated media and of those developed in our laboratory and used in cell treatment