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10.1002/acn3.50838 Ann Clin Transl Neurol

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Then, the nuclear pellet was homogenized on ice for 1 h in buffer B [20 mM HEPES (pH 7.9), 0.5 mM dithiothreitol, 1.2 mM MgCl2, 25% glycerol, 0.2 mM EDTA, 420 mM NaCl, 0.5 mM PMSF and 1% protease inhibitor cocktail (P8340, Sigma)]