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Real-time-qPCR samples were preheated at 95C for 10 min, followed by 40 cycles of denaturation at 95C for 15 s and annealing/extension at 60C for 1 min

Differential mechanism of block of palmitoyl lysophosphatidylcholine and of palmitoylcarnitine on inward rectifier K + channels of guinea-pig ventricular myocytes