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In Echinodermata (which share the common ancestor with Chordata), the radial glia emerged, which signals the appearance of the layered cytoarchitecture

[DOI] [PMC free article] [PubMed] [Google Scholar] Brusselle G., Germinaro M., Weiss S., Zangrilli J

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Moreover, it was also shown that, as a calcium-binding protein, the Fh8 tag and Fh8-fused proteins can be eluted by using a calcium chelating agent, such as EDTA