glutathione magnetic beads sigma Millipore High Capacity Agarose Beads, (1:1 suspension in a 30% ethanol solution) 1 ml glutathione peroxidase XpressAffinty protein A/G magnetic beads/

Glutathione biosynthesis can be affected by biochemical individuality and/or dietary factors

Acknowledgments We acknowledge Indonesian Cosmetic Dermatology Study Group, who supported the study

3Conclusion The complex interaction between various cytosolic and intranuclear components with NRF-2, although better understood, continues to encounter a lack of information, leading us to clearly identify under which conditions its action is activated or suppressed by means of various, already identified clinical situations

After the plates were treated with 1D228 (10, 100 and 1000 nM) or DMSO (0.32% or 0.64%) for 48 h at 37 C, then cultuerd in 10% FBS medium, the colonies were fixed with 4% paraformaldehyde and stained with 1% crystal violet solution for 20 min