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Kui-Yang Zheng, [email protected] These authors have contributed equally to this work This article was submitted to Cancer Immunity and Immunotherapy, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The 4.3 kb adenoviral fragment contains early region 1 (E1) transcription units E1A and E1B, integrated into chromosome 19 of the host genome 61,62 , enabling viral gene expression and therapeutic protein overexpression under viral, mammalian, or synthetic promoters