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Experimental animal models of class Mammalia, order Rodentia comprising healthy animals of both sexes that have been exposed to basal or standard laboratory diets without supplementation of selenium for weight maintenance will be considered a control

2001 Mar;131(3s):1010S-5S

Small timing variations (a few hours earlier or later on your chosen day) won't significantly impact effectiveness

However, in the testicular tissue collected from the diabetes control group of rats, cell stability in the mitotic phase, congestion, vacuolization or edema in the interstitial tissue, spilled cells in the tubules, and cell infiltration were all observed