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LC-MS/MS is widely regarded as a gold standard due to its inherent sensitivity, specificity, and capability for simultaneous quantification of multiple vitamins and their metabolites from limited sample volumes, which is particularly advantageous in pediatric CF patients (Konieczna et al., 2016

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The second reaction was initiated through the addition of the remaining 10% (10 l) reaction volume containing GSH and GST and the same plate reader method as used for one-step reactions was applied to follow the reaction progress