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The absorbance of the yellow product was measured at 412 nm (or Ex/Em 405/480 nm for the fluorometric version)

signalling was activated by further addition of 10 g per mL of -mouse IgG/IgM secondary antibody (Dianova, #115-005-068) for the given time points

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Vitamin B12 is involved in several processes that directly affect how your mind works: It builds and maintains myelin