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10.1002/jcp.25826 191

Such trends might have led to statistically significant differences if a larger senior feline cohort had been used

The details of the primer, used in this study for PCR amplification of MT genes, is shown below: qPCR analysis The MT gene quantification was conducted using the SYBR Green method with a StepOnePlus Real-Time PCR system from Applied Biosystems, USA