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In the case of inhaled materials, animal data have been taken from studies that consist of exposing rodents to chemicals in whole-body or nose-only systems, such as rodent acute (US EPA, 1998) inhalation toxicity studies from which no observed effect concentration (NOEC) or no observed adverse effect concentration (NOAEC) are derived to inform human health inhalation risk assessments ( In vivo studies have been used in risk assessment for many decades

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Disruption of key proteins due to oxidative stress leads to melanocyte detachment from keratinocytes, which is essential for their survival, with disrupted cell adhesion proteins such as E-cadherin exacerbating melanocyte loss and disease progression 113