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Moreover, THHE reduces the release of pro-inflammatory cytokines (IL-1, IL-1, and MMP-3), and elevates the expression of anti-inflammatory mediators (IL-4 and IL-10)

This technique relies on reacting the SFN enantiomers with a chiral derivatization reagent, creating diastereomers that can be separated using chromatography with achiral stationary phases [129]