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Applichem GmbH, Germany) and 1% BSA in PBS, for 30 min at 4 C, and were immediately scraped into lysis buffer, to reveal the endocytosed pool of molecules

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These include verifying in vivo bioavailability, tissue-specific targeting, and the durability of gene expression changes

As with SIRS, CARS is a complex immune response involving anti-inflammatory and immunosuppressive mechanisms that are able to restore homeostasis after injury and SIRS or, if unchecked leads to a state of profound immunosuppression, predisposing the individual to infections [52, 55]