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While there is evidence for nLD biogenesis in the INM of U2OS cells, it is uncertain whether this pathway is responsible for formation of nLDs in other mammalian cells or works in conjunction with the eLD pathway for nLD formation in hepatocytes (see below)

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Sigma-Aldrich, USA), and an RNS-sensitive fluorescence indicator, 3-amino, 4-aminomethyl-2,7-difluorofluorescein diacetate (DAF-FM DA