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Denuded oocytes were fixed in 4% PFA for 30 min, followed by incubation in PBS containing 3 mg/ml BSA and 7.5 mg/ml glycine for 15 min

A general rule regarding buffers is that the pH of the buffer solution should be within 1.0 pH unit of the pK a to provide appropriate buffering capacity

Evaluating how structural properties influence the performance of proximity metrics in single cell RNA-seq data