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In mouse brain, DR led to a 7-fold increase in GPD2 persulfidation, but no significant increase in GPD1 persulfidation ( H 2 S has been shown to be essential for DR-induced longevity ( 2 S-generating enzyme MPST, resulting in increased mitochondrial ETC function and increased exercise endurance (Sprenger et al., 2025), as the H 2 S generated by the metabolism of endogenous skeletal muscle ergothioneine was shown to increase mitochondrial ETC activity through the transfer of electrons from H 2 S to ubiquinone (Landry et al., 2018)