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Recombinant proteins are usually expressed with an affinity tag that facilitates binding of the protein to an affinity matrix and results in high yield purification of proteins (usually above 90% yield) by exploiting avidity between the tag and affinity matrix

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[21] [22] At the age of thirteen, Pattinson joined the Barnes Theatre Company, a local amateur theatre group, after his father encouraged him to participate to help overcome his shyness