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We expressed and purified ABHD11-Flag in HEK293T cells and confirmed its hydrolase activity using a generic esterase substrate, p-nitrophenol acetate (Fig

Melndez-Hevia E, De Paz-Lugo P, Cornish-Bowden A, Crdenas ML

Saunders company, 1975)

Rockville (2001) Guidance for industry bioanalytical method validation, US Department of Health and Human Services, US Food and Drug Administration (FDA), Center for Drug Evaluation and Research (CDER), Center for Biologics Evaluation and Research (CBER)