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For instance, (Shi et al., 2023), developed a BBB-on-a-chip model using microvascular endothelial cells, pericytes, and mast cells to mimic the glioma microenvironment, enabling synergistic effect evaluation from traditional Chinese medicine phytochemicals to improve drug delivery and efficacy (Garcia et al., 2023) created a model combining 3 cell types: brain cells, human astrocytes-hippocampal (Ha-h), and Human brain vascular pericytes (HBVP), to test permeability and internalization of PLGA-encapsulated ferulic acid

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