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After initial growth to an OD600 of 0.8, cells were induced using 0.4 mM isopropyl -D-1-thiogalactopyranoside (IPTG) and cultured at 20C for 16 hours

A hydrogel microsphere with micelle microfluidic properties contains a ketone enol thiol-bridged nanoscale secondary structure, which downregulates TfR1 expression through metal chelation and increases the expression of CDGSH iron sulfur domain-containing protein 1 (CISD1), thereby restoring mitochondrial iron homeostasis and promoting cartilage repair (170)

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