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mobile phase is the triethylamine buffer solution (8ml of phosphate, 15ml of triethylene amine, 1500ml of water, pH adjusted to 5.4) - tetrahydrofuran(THF) mixture, the time gradient is listed in the following Table 2:Table 2 HPLC gradient time table The content of D-isomer of L-3-cyano-2-hydroxypropyl trimethyl ammonium detected is shouwed in table 3Table 3 The results of the content of D-isomer of L-3-cyano -2 -hydroxypropyl Trimethyl Ammonium Step1 Preparation of L-3-chloro-2-hydroxypropyl trimethylammonium Firstly, the content of L-isomer of S-epichlorohydrin was detected according to the method of example 1 above

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Int J Epidemiol 18:858867 Li XS, Wang Z, Cajka T et al (2018) Untargeted metabolomics identifies trimethyllysine, a TMAO-producing nutrient precursor, as a predictor of incident cardiovascular disease risk

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