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The freeze-drying and spray-drying processes used to make instant coffee dont appear to significantly affect chlorogenic acid levels, but the method of preparing fresh coffee does

J Ethnopharmacol 127:2631

For the affinity chromatography at low pressure, nonrigid gels with large particle size are generally used as support materials while materials with small, rigid particles or synthetic polymers which are stable under high pressure and flow rates are used in high performance affinity applications [8,12]