Mice were housed under a 12/12-h light/dark cycle with ad libitum access to food and water
The main advantages of nanopore sequencing are: small size (the devices are portable and usable in the field), no prior need of DNA amplification, no apparent limit of DNA strand length [95] (ultra long reads, showing variations within the whole genome), high throughput [91], no bias in sequencing quality with read length (useful in analyzing highly repetitive regions of the DNA) [49], distinguishing 5hmC signals from 5mC signals and allele-specific methylation [6], low prices (and currently going down, becoming comparable to those of short-read sequencing through bisulfite techniques) [25, 71, 96], fast sample preparation and analysis (the full process of complete methylation analysis only takes a few hours, and depending on the GPU power of the computer that is used it could sometimes take only 13 h, which is promising for real-time applications in cancer) [81], high accuracy ranging from 96.398.8% [97]
Precautions Should not replace a varied and balanced diet and a healthy lifestyle
Methods: 20 healthy controls (HC) (33 years
