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Under idealized biochemical accounting, free palmitate is commonly estimated to yield approximately 106 ATP equivalents after subtracting the two-ATP-equivalent activation cost, whereas already activated palmitoyl-CoA is often accounted as approximately 108 ATP equivalents under the same assumptions because the activation step has already been performed

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Next, the membranes were saturated with a rapid blocking buffer (NCM Biotech, Suzhou, China) for 20 min

Geng Q, Xu Y, Hu Y, Wang L, Wang Y, Fan Z, Kong D (2024) Progress in the application of Organoids-On-A-Chip in diseases