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Funding Research reported in this publication was funded by the NHLBI Recipient Epidemiology and Donor Evaluation Study- III (REDS-III), which was supported by NHLBI contracts NHLBI HHSN2682011-00001I, -00002I, -00003I, - 00004I, 00005I, -00006I, -00007I, -00008I, and -00009I, as well as funds from the the National Institute of General and Medical Sciences (RM1GM131968 to ADA), NHLBI R01HL146442 and R01HL149714 (ADA), R01HL148151 (ADA, JCZ), the Boettcher Webb-Waring Investigator Award (ADA) and a Shared Instrument grant by the National Institute of Health (S10OD021641)

Critical step: Direct the water stream against the inside wall of the vial, not directly onto the powder