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Supernatants were diluted with ChIP dilution buffer (50 mM Tris-HCl [pH 8.0], 167 mM NaCl, 1.1% Triton X-100, 0.11% NaDoc, 200 nM PMSF), precleared with normal rabbit IgG (Cell Signaling Technology, #2729) or normal mouse IgG (Santa Cruz, sc-2025), and incubated with Protein G agarose beads (Thermo Scientific, 20398) pre-washed in 3.125 mg/mL BSA/ChIP dilution buffer for 2 h at 4 C

Concentrations of TIMP-1 were 15% higher ( p = 0.074) among PTSD participants on SSRIs ( n = 13) compared to participants not on SSRIs ( n = 12)

2012;4:127ra37