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This observation was in line with the immunofluorescence analysis, which indicated that in fibrotic-like assembloids some hepatocytes (HNF4A + GFP ) were positive for the cholangiocyte marker KRT19, and opened lumina, resembling the polarity of simple ductal epithelium, suggestive of potential hepatocyte-to-duct transdifferentiation (Extended Data Fig

Peer review Peer review information Nature Cardiovascula r Research thanks the anonymous reviewers for their contribution to the peer review of this work