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While The Cancer Genome Atlas (TCGA) initially identified four molecular subgroups of GBM (classical, neural, pro-neural, and mesenchymal) based on dominant genes expressed using transcriptional profiling data of bulk tumor specimens [13], at single-cell RNA-sequencing resolution, a single tumor actually consists of a heterogeneous mixture of cells representing all of the different GBM subgroups [14]

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doi: 10.1177/0748233718763517