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[46] did not estimate a sample size required for a study of this nature (a survey study). [48] In response, Schultz [49] stated that, given that calculating a studys appropriate sample size requires some foreknowledge of the size of the expected effect, the appropriate sample size could not have been calculated prior to initiation of the study

Prog Retin Eye Res (2016) 51:140

Catabolism and Anabolism in TCA Cycle The TCA cycle integrates both catabolic and anabolic biochemical reactions of nutrients and intermediary metabolites