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IFNy inhibits replication of ectromelia, vaccinia, and herpes simplex virus- 1 (HSV-1) viruses in mouse macrophages correlated with the cells' production of NO

To visualize small-molecule metabolites localized within TLS, we used matrix-assisted laser desorption/ionization time of flight imaging mass spectrometry and found significant accumulation of glutathione (reduced form: glutathione [GSH], oxidized form: glutathione disulfide [GSSG]) within TLS (Figure 1C)