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Conventional photo-cross-linking strategies can be used to identify transient interactions, but these approaches often suffer from high background due to the cross-linked bait proteins

The cells were cultured in streptomycin (50 g/mL), penicillin (1%, 50 IUmL 1 ), and RPMI-1640 medium (10% fetal bovine serum (FBS)) in a CO 2 -filled humidified incubator at 37 C

Plant dry mass was calculated after drying them at 80 C in a hot air oven until constant weight is attained 55

The proposed mechanisms remain largely theoretical, and the pathway from glutamine to arginine to nitric oxide is indirect and influenced by numerous other factors, including the presence of competing metabolic pathways and individual variations in enzyme activity