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MRD cells were also generated in vivo, by treating mouse xenografts of the 105K Tsc2 / RCC line in syngeneic C57BL/6J mice with rapamycin (3 mg/kg, intraperitoneally [IP] 3 times/wk for 1 month, MRD525), or the bi-steric inhibitor RMC-6272 (8 mg/kg, IP once/wk for 1 month, MRD639), followed by 2 months of treatment cessation (Figure 1A)

Energy metabolism and lipid metabolism are intimately connected, with many of the dysregulated lipids identified in these patients being essential components of cellular membranes and having significant roles in energy metabolism (Hu et al., 2022)

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