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Stimulation with 6-hydroxydopamine (6-OHDA) markedly downregulates nuclear factor erythroid 2related factor 2 (NRF2) expression in PC-12 cells, elevates Fe 2+ and reactive oxygen species (ROS) levels, suppresses glutathione peroxidase 4 (GPX4), promotes both ferroptosis and apoptosis, inhibits cell proliferation, and causes neuronal damage

Lysate from His-tagged proteins were mixed with Ni-NTA beads (Qiagen) 2 h, and washed with lysis buffer supplemented with 25 mM imidazole

J Exp Bot 70(18):48354848

In the study by iauskait and his team, highly metastatic human colon carcinoma cells were treated with sulforaphane, a bioactive compound found in cruciferous vegetables, in combination with the FOLFOX regimen (a combination of 5-FU, leucovorin, and oxaliplatin) to demonstrate an additive anticancer effect