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Important Disclaimer: AOD-9604 is sold for research purposes only and is not FDA-approved for human use

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While singly-housed animals may experience stress that impacts metabolism, body weight, and adiposity 65,66,67,68,69 , the singly-housed animals in this study were treated identically (to the best of the experimenters ability) to minimize and equalize stress across all animals and reduce confounding variables

Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas