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Initially, cells were infected with four different zoospore (#87) loads, 0 MOI, 0.5 MOI, 1 MOI and 2 MOI, and incubated at 20C for 4 days

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The cells were treated with FAC for 24 h, washed with phosphate buffer solution (PBS), and stained with Annexin V pacific blue (BIOL-640918, Biolegend) for 15 min in the dark, then stained with PI (Invitrogen) before detection