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The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3 M Empore)

DAB horseradish peroxidase color development kit (ZSGB-bio, cat: ZLI-9018) was utilized to visualize the brain tissue sections, which were subsequently counterstained with hematoxylin, and observed under a microscope

A thiol-based intramolecular redox switch in four-repeat tau controls fibril assembly and disassembly

Serotonin acts together with dopamine to modulate octopaminergic and tyraminergic neurons, and serotonin also acts on other neuronal targets to regulate behavior