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Ruan et al., 2024

319 This signaling pathway begins when TGF- binds to type I and type II serine/threonine kinase receptors, triggering the phosphorylation and activation of receptor-regulated SMAD proteins (SMAD2 and SMAD3)

The residual hydrogen peroxide is determined colorimetrically by an enzymatic reaction that involves the conversion of 3,5-dichloro-2-hydroxybenzensulphonate to a colored product